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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

ENTPD5 (E5U3V) Rabbit mAb #73612

Filter:
  • WB
  • IHC
Western Blotting Image 1: ENTPD5 (E5U3V) Rabbit mAb
Western blot analysis of extracts from JHH-5 and Caki-1 cells using ENTPD5 (E5U3V) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). Low expression of ENTPD5 protein in Caki-1 cells is consistent with the predicted expression pattern.

To Purchase # 73612

Cat. # Size Qty. Price Ships
73612T 20 µl
$182
73612S 100 µl
$402

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 47
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IHC-Immunohistochemistry 
Species Cross-Reactivity Key:
  • H-Human 

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunohistochemistry (Paraffin) 1:150 - 1:600

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

ENTPD5 (E5U3V) Rabbit mAb recognizes endogenous levels of total ENTPD5 protein. Non-specific staining was observed in pancreatic islets by immunohistochemistry.

Species Reactivity:

Human

The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

Species predicted to react based on 100% sequence homology:

Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human ENTPD5 protein.

Background

ENTPD5 is a member of the ectonucleoside triphosphate diphosphohydrolase family, which plays a role in the metabolism of nucleotides, specifically by hydrolyzing nucleoside diphosphates (NDPs) to their respective nucleoside monophosphates. It is an integral membrane protein that is found in the endoplasmic reticulum (ER) membrane, where it acts as a nucleoside diphosphatase, catalyzing the hydrolysis of nucleoside diphosphates, such as UDP (uridine diphosphate), GDP (guanosine diphosphate), and CDP (cytidine diphosphate), into their respective nucleoside monophosphates. This enzymatic activity is essential for the regulation of nucleotide levels in the ER. ENTPD5's role in nucleotide metabolism and regulation suggests that it may play a part in various cellular processes, including glycosylation, protein folding, and signal transduction within the ER (1). Aberrant expression or mutations in ENTPD5 have been suggested to activate oncogenic activity in several cancers (2,3).
For Research Use Only. Not For Use In Diagnostic Procedures.
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