Render Target: STATIC
Render Timestamp: 2024-12-26T11:36:46.338Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-08-01 15:25:42.843
Product last modified at: 2024-10-25T12:45:08.738Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

EphB6 Antibody #12560

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 125
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    EphB6 antibody recognizes endogenous levels of total EphB6 protein.

    Species Reactivity:

    Human

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala927 of human EphB6 protein. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    EphB6 is a kinase-defective receptor and member of the ephrin-B family of transmembrane proteins (1). Although lacking kinase activity, EphB6 can regulate cellular functions through its interaction with adaptor proteins and other Eph family members (2). In hematopoietic cells, EphB6 is specifically expressed in the T cell population (3) and functions as an important regulator of T cell receptor (TCR) mediated signaling. Upon binding with its ephrin-B1 or ephrin-B2 ligand, EphB6 modulates TCR activity through inhibition of JNK signaling, reduction of CD25 expression, and decreased IL-2 secretion (4). Reduced levels of cell proliferation and cytokine secretion are seen in EphB6 knock-out mice relative to wild type (5). In conjunction with EphB3 receptor activation, EphB6 suppresses Fas receptor induced apoptosis by triggering the Akt activation pathway (6). Research indicates that decreased EphB6 expression is associated with a higher degree of metastasis in various cancers, including breast cancer (7), lung cancer (8), and neuroblastoma (9). EphB6 is thought to reduce cancer invasiveness through its effect on cell adhesion and migration. Following EphrinB1 ligand binding, EphB6 is phosphorylated by kinases such as Src and another active EphB kinase (2, 10, 11). Phosphorylated EphB6 forms a stable complex with Cbl and initiates Cbl inhibition of cell adhesion (2,11). EphB6 regulates signal transduction through direct interaction with other active Eph receptor kinases, sequestering these EphB6-bound receptors and inhibiting typical signal transduction function (12).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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