Render Target: STATIC
Render Timestamp: 2024-12-20T11:25:07.687Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-09-30 02:00:06.875
Product last modified at: 2024-11-22T12:45:10.905Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. EPHX2 (F9Z3M) Rabbit mAb
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

EPHX2 (F9Z3M) Rabbit mAb #37117

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 60
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Simple Western™ 1:10 - 1:50
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    EPHX2 (F9Z3M) Rabbit mAb recognizes endogenous levels of total EPHX2 protein. This antibody also recognizes a non-specific band of unknown origin at 140 kDa.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val380 of human EPHX2 protein.

    Background

    Soluble epoxide hydrolase 2 (EPHX2, sEH) is an enzyme member of the α/β hydrolase superfamily important for epoxide metabolism, especially those derived from fatty acids including epoxyeicosatrienoic acids (EETs). EPHX2 contains two functional domains connected by a proline-rich linker: a carboxy-terminal domain is responsible for its epoxide hydrolase activity and an amino-terminal phosphatase domain (1). EPHX2 is widely expressed in the central nervous system and peripheral tissue types, with highest expression found in the kidney and liver (1,2). Mutations in the EPHX2 gene have been linked to familial hypercholesterolemia due to its impact on plasma cholesterol levels (3). Studies have shown that suppression of EPHX2 activity through selective inhibitors promotes anti-inflammatory, cardioprotective, and neuroprotective effects through increased circulating EET levels, implicating EPHX2 as a therapeutic target for several disease conditions (4-6).

    Database Links

    UniProt ID: P34913


    Entrez-Gene Id: 2053


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    For Research Use Only. Not For Use In Diagnostic Procedures.
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