Render Target: STATIC
Render Timestamp: 2024-11-29T11:18:10.880Z
Commit: cd2fae6ca3f811b1ddb1df24ac291ed56d5d501b
XML generation date: 2024-09-20 06:17:32.949
Product last modified at: 2024-09-13T07:01:03.242Z
1% for the planet logo
PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

Estrogen Receptor β Antibody #5513

Filter:
  • WB

Inquiry Info. # 5513

Please see our recommended alternatives.

    Supporting Data

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 52, 55, 63
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Estrogen Receptor β Antibody detects endogenous levels of total Estrogen Receptor β protein. This antibody is predicted to cross-react with all Estrogen Receptor β isoforms. This antibody does not cross-react with Estrogen Receptor α.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human Estrogen Receptor β1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Estrogen Receptor β (ER β) is a member of the nuclear receptor superfamily of transcription factors and was discovered to be encoded by a gene (ESR2) distinct from that encoding Estrogen Receptor α (ER α) (1,2). While studies have revealed that alternative splicing generates mutiple isoforms of ER β that differ at their respective C-termini and in tissue distribution, ER β1 is believed to be the longest and only fully functional isoform (3,4). Indeed, it has been reported that shorter isoforms of ER β (ER β2, β4, and β5) can heterodimerize with ER β1 and enhance its transcriptional activity in an estradiol-dependent manner (4). ER β is expressed in a wide range of normal and malignant tissues, many of which coexpress ER α. It is proposed that ER β has an antiproliferative role, perhaps through heterodimerization with ER α and repression of its transcriptional activity at estrogen response elements (5,6). Recent studies have revealed that expression of ESR2 is subject to epigenetic regulation and that loss of ER β expression positively contributes to epithelial-mesenchymal transition and enhanced invasiveness in prostate cancer (7,8). ER β has also been found to be negatively regulated at the posttranslational level through phosphorylation of its AF-1 domain, which promotes its ubiquitin-dependent proteasomal degradation (9,10).
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.