Estrogen Receptor β (E2K7F) Rabbit mAb #89954
- WB
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Transfected Only |
| MW (kDa) | 60 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
Estrogen Receptor β (E2K7F) Rabbit mAb recognizes overexpressed levels of total estrogen receptor β protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu10 of human estrogen receptor β protein.
Background
Estrogen receptor β (ERβ) is part of the nuclear receptor superfamily related to estrogen receptor α (ERα) and encoded by the ESR2 gene on chromosome 14 (1,2). Much like ERα, ERβ contains two activator functions, one in the N-terminal domain and one in the ligand binding domain. Both proteins have highly homologous DNA-binding domains and can synergistically regulate transcription (2,3). ERβ is tightly regulated by various mechanisms including estrogen binding, post-translational modifications, alternative splicing, circadian controls, and DNA methylation of the gene (4,5). The role of ERβ in various cancer types is controversial, but it is generally thought to be a tumor suppressor. Absence of ERβ has been shown to be a marker of poor prognosis in breast cancer and is correlated with tamoxifen resistance (6,7). In prostate cancer, ERβ can induce apoptosis, but its expression seems to be lost in higher grade tumors (8,9).
- Enmark, E. et al. (1997) J Clin Endocrinol Metab 82, 4258-65.
- Kumar, R. et al. (2011) J Amino Acids 2011, 812540.
- Jia, M. et al. (2015) Best Pract Res Clin Endocrinol Metab 29, 557-68.
- Moore, J.T. et al. (1998) Biochem Biophys Res Commun 247, 75-8.
- Swedenborg, E. et al. (2009) Cell Mol Life Sci 66, 3873-94.
- Omoto, Y. et al. (2001) Cancer Lett 163, 207-12.
- Hopp, T.A. et al. (2004) Clin Cancer Res 10, 7490-9.
- McPherson, S.J. et al. (2010) Proc Natl Acad Sci USA 107, 3123-8.
- Fixemer, T. et al. (2003) Prostate 54, 79-87.
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