Render Target: STATIC
Render Timestamp: 2024-12-26T11:03:36.950Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-09-30 01:59:09.516
Product last modified at: 2024-10-28T17:00:16.661Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Estrogen Receptor β (E2K7F) Rabbit mAb #89954

Filter:
  • WB

    Supporting Data

    REACTIVITY H
    SENSITIVITY Transfected Only
    MW (kDa) 60
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Estrogen Receptor β (E2K7F) Rabbit mAb recognizes overexpressed levels of total estrogen receptor β protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu10 of human estrogen receptor β protein.

    Background

    Estrogen receptor β (ERβ) is part of the nuclear receptor superfamily related to estrogen receptor α (ERα) and encoded by the ESR2 gene on chromosome 14 (1,2). Much like ERα, ERβ contains two activator functions, one in the N-terminal domain and one in the ligand binding domain. Both proteins have highly homologous DNA-binding domains and can synergistically regulate transcription (2,3). ERβ is tightly regulated by various mechanisms including estrogen binding, post-translational modifications, alternative splicing, circadian controls, and DNA methylation of the gene (4,5). The role of ERβ in various cancer types is controversial, but it is generally thought to be a tumor suppressor. Absence of ERβ has been shown to be a marker of poor prognosis in breast cancer and is correlated with tamoxifen resistance (6,7). In prostate cancer, ERβ can induce apoptosis, but its expression seems to be lost in higher grade tumors (8,9).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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