Render Target: STATIC
Render Timestamp: 2024-11-14T11:00:21.891Z
Commit: 3c1f305a63297e594ac8d7bb5424007d592d68be
XML generation date: 2024-08-01 15:27:46.321
Product last modified at: 2024-05-30T07:03:10.108Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

ETS-1 Antibody #6258

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Inquiry Info. # 6258

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    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 52
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    ETS-1 Antibody recognizes endogenous levels of total ETS-1 protein.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser179 of human ETS-1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    ETS-1 is a proto-oncoprotein that belongs to the E26 Transformation-specific Sequence (ETS) family of transcription factors that share a unique and highly conserved DNA binding domain (1). ETS-1 plays important roles in vascular development and angiogenesis (2), and vascular inflammation and remodeling (3). The target genes of ETS-1 include receptor tyrosine kinases, MMPs, and cell adhesion molecules (4-6). In addition, ETS-1 is involved in regulation of energy metabolism in cancer cells (7). ETS-1 activity is regulated by two different types of phosphorylation sites. While phosphorylation at a cluster of serine residues in the exon VII domain by CaMKII inhibits ETS-1 DNA binding activity (8), phosphorylation at its Thr38 site by Ras activates ETS-1 (9).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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