Render Target: STATIC
Render Timestamp: 2024-12-16T14:02:50.925Z
Commit: 611277b6de3cd1bb065350b6ef8d63df412b7185
XML generation date: 2024-08-01 15:25:40.308
Product last modified at: 2024-10-28T11:45:43.569Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

EVL Antibody #12536

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 52
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    EVL Antibody recognizes endogenous levels of total EVL protein.

    Species Reactivity:

    Human

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser362 of human EVL protein. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Ena/VASP-like (EVL) protein is a member of the Ena/VASP family and is involved in actin-associated cytoskeleton remodeling and cell polarity activities including axon guidance and lamellipodia formation in migrating cells (1,2,3). The EVL protein sequence contains an N-terminal EVH1 domain, a Pro-rich SH3 binding domain, and a C-terminal EVH2 domain. EVL domain interactions with G- and F-actin mediates actin nucleation and polymerization (4). Research studies have shown that EVL also regulates DNA repair by direct interaction with RAD51 (5). EVL may function in the DSB repair pathway through the EVH2 domain, which possesses DNA-binding and RAD51 binding activity, thereby coordinating homologous DNA recombination (6,7). Research studies have shown EVL expression is up-regulated in human breast cancer associated with clinical stages and may be implicated in invasion and/or metastasis of human breast cancer (8).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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