EXO1 (E6P2B) Rabbit mAb #63862
- WB
Supporting Data
| REACTIVITY | H M |
| SENSITIVITY | Endogenous |
| MW (kDa) | 120 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
EXO1 (E6P2B) Rabbit mAb recognizes endogenous levels of total EXO1 protein.
Species Reactivity:
Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro377 of human EXO1 protein.
Background
Mismatch repair (MMR), a conserved process that involves correcting errors made during DNA synthesis, is crucial to the maintenance of genomic integrity. MMR requires recognition of a base mismatch or insertion/deletion loop by a MutS homolog followed by recruitment of a MutL heterodimeric complex consisting of MLH1 and PMS1 (MutL-γ), PMS2 (MutL-α), or MLH3 (MutL-γ). Other factors required for MMR in eukaryotes are EXO1, PCNA, RFC, RPA, DNA polymerases, and DNA ligases (1). In addition to its role in MMR, the nuclease EXO1 functions in DNA double-strand break repair, replication stress response, nucleotide excision repair (NER), and telomere maintenance (2). AMPK phosphorylates EXO1 at Ser746 in response to replication stress and subsequent calcium elevation to avoid replication fork resection (3). Mutations in the EXO1 gene are associated with multiple human cancers (4).
Limited Uses
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