Render Target: STATIC
Render Timestamp: 2024-12-20T11:04:43.885Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-09-30 01:55:22.215
Product last modified at: 2024-12-17T18:54:29.026Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

EZH1 (D7D5D) Rabbit mAb #42088

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H Mk
    SENSITIVITY Endogenous
    MW (kDa) 95
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    EZH1 (D7D5D) Rabbit mAb recognizes endogenous levels of total EZH1 protein. This antibody does not cross-react with EZH2 protein.

    Species Reactivity:

    Human, Monkey

    The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

    Species predicted to react based on 100% sequence homology:

    Pig

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human EZH1 protein.

    Background

    The polycomb group (PcG) proteins are involved in maintaining the silenced state of multiple developmentally regulated genes and contribute to the maintenance of cell identity, cell cycle regulation, and oncogenesis. Enhancer of zest homolog 1 (Ezh1), is a member of this large protein family and a subunit of the polycomb repressor complex 2 (PRC2), also containing SUZ12 and EED. Ezh1 and its paralog Ezh2 are mutually exclusive catalytic subunits of the PRC2 complex, which functions to mono-, di-, and tri-methylated Lys27 on histone H3, a mark that is associated with transcriptional repression. While EZH1 is less abundant than EZH2, it is still required for cell identity and self-renewal of embryonic stem cells (1,2). Ezh1 is also required for hematopoietic stem cell maintenance and functions to prevent a senescence-like cell cycle arrest (3). Ezh1 is required for myogenic differentiation and hepatocyte homeostasis and regeneration (4,5). While many studies have implicated Ezh2 in multiple types of cancer, a potential role for Ezh1 is less understood. However, several studies have shown dual inhibitors of Ezh1/Ezh2 to be more effective than Ezh2-specific inhibitors in treatment of multiple myeloma, prostate cancer, diffuse large B-cell lymphoma, and leukemia, suggesting an important role for Ezh1 in cancer (6-8).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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