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Product last modified at: 2025-03-14T18:30:11.281Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

Ezh2 (AC22) Mouse mAb #3147

Filter:
  • WB
Western Blotting Image 1: Ezh2 (AC22) Mouse mAb
Western blot analysis of extracts from various cell lines using Ezh2 (AC22) Mouse mAb.

To Purchase # 3147

Cat. # Size Qty. Price
3147T 20 µl
$152
3147S 100 µl
$336

Supporting Data

REACTIVITY H M R Mk
SENSITIVITY Endogenous
MW (kDa) 98
Source/Isotype Mouse IgG1
Application Key:
  • WB-Western Blotting 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Mk-Monkey 
  • Related Products
  • Conjugates

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #44495.

Protocol

Specificity / Sensitivity

Ezh2 (AC22) Mouse mAb detects endogenous levels of total Ezh2 protein.

Species Reactivity:

Human, Mouse, Rat, Monkey

Source / Purification

Monoclonal antibody is produced by immunizing animals with Ezh2 recombinant protein containing human Ezh2 residues 351-453.

Background

The polycomb group (PcG) proteins are involved in maintaining the silenced state of several developmentally regulated genes and contribute to the maintenance of cell identity, cell cycle regulation, and oncogenesis (1,2). Enhancer of zeste homolog 2 (Ezh2), a member of this large protein family, contains four conserved regions including domain I, domain II, and a cysteine-rich amino acid stretch that precedes the carboxy-terminal SET domain (3). The SET domain has been linked with histone methyltransferase (HMTase) activity. Moreover, mammalian Ezh2 is a member of a histone deacetylase complex that functions in gene silencing, acting at the level of chromatin structure (4). Ezh2 complexes methylate histone H3 at Lys9 and 27 in vitro, which is thought to be involved in targeting transcriptional regulators to specific loci (5). Ezh2 is deregulated in various tumor types, and its role, both as a primary effector and as a mediator of tumorigenesis, has become a subject of increased interest (6).

Pathways

Explore pathways related to this product.


For Research Use Only. Not For Use In Diagnostic Procedures.
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