Render Target: STATIC
Render Timestamp: 2024-12-20T11:47:29.420Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-08-01 15:28:34.225
Product last modified at: 2024-12-09T20:30:08.613Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

EZHIP Antibody #96227

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 80
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    EZHIP Antibody recognizes endogenous levels of total EZHIP protein.

    Species Reactivity:

    Human

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala495 of human EZHIP protein. Antibodies are purified by peptide affinity chromatography.

    Background

    EZH inhibitory protein (EZHIP), also called CXorf67 or Catacomb, is a protein discovered in aggressive posterior fossa A ependymomas (1-3). EZHIP contains a highly conserved sequence surrounding a methionine near its C-terminus which mimics the activity of the oncohistone protein H3K27M, and allows it to bind EZH2 and inhibit PRC2 function to lower H3K27me2/3 levels (2-4). EZHIP has also been shown to play a regulatory role in developing sperm and oocytes (5).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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