Render Target: STATIC
Render Timestamp: 2024-12-20T11:23:17.981Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-09-30 01:59:49.341
Product last modified at: 2024-10-22T13:00:17.071Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

FADS1 (F1D2A) Rabbit mAb #10076

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 43
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Simple Western™ 1:50 - 1:250
    Immunoprecipitation 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    FADS1 (F1D2A) Rabbit mAb recognizes endogenous levels of total FADS1 protein. This antibody does not cross-react with FADS2 protein.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe100 of human FADS1 protein.

    Background

    Fatty acid desaturase 1 (FADS1), also known as delta-5 desaturase, is essential for the biosynthesis of highly unsaturated fatty acids (HUFAs) from linoleic acid (LA) and α-linolenic acid (ALA), both of which are polyunsaturated fatty acids (PUFAs). In addition, FADS1 converts NADH to NAD+. Studies show that FADS1 and fatty acid desaturase 2 (FADS2) thus enable NAD+ recycling for glycolysis to support cell viability when aerobic respiration is acutely suppressed (1,2). Furthermore, FADS1 and ELOVL5 expression is enhanced in mesenchymal-type gastric cancer cells, sensitizing the cancer cells to ferroptosis. On the contrary, FADS1 and ELOVL5 expression is inhibited in intestinal-type gastric cancer cells, leading to resistance to ferroptosis (3).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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