Render Target: STATIC
Render Timestamp: 2024-11-21T12:51:27.041Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-09-30 01:55:24.137
Product last modified at: 2024-11-20T12:45:11.338Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

Fer (5D2) Mouse mAb #4268

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H M
    SENSITIVITY Endogenous
    MW (kDa) 95
    Source/Isotype Mouse IgG1
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Simple Western™ 1:50 - 1:250
    Immunoprecipitation 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Fer (5D2) Mouse mAb detects endogenous levels of total Fer protein. This antibody does not cross-react with other related proteins.

    Species Reactivity:

    Human, Mouse

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a truncated recombinant Fer protein.

    Background

    Fer is a nonreceptor protein-tyrosine kinase of the Fes/Fps family. Like many other cytoplasmic tyrosine kinases, Fer contains a long amino-terminal domain, a central SH2 domain, and a carboxy-terminal kinase domain. Its amino-terminal domain is responsible for protein oligomerization as well as interaction with cytoskeletal proteins. Fer is ubiquitously expressed in a wide variety of cell and tissue types, and is localized to both cytoplasm and nucleus (1). Tyrosine kinase activity of Fer can be stimulated by growth factors and cytokines (2,3). After activation, Fer can further activate various downstream signaling components including Stat3 (3). Fer plays an important role in regulation of cell movement, oncogenesis and inflammation (4).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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