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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

Fer (5D2) Mouse mAb #4268

Filter:
  • WB
  • IP
Western Blotting Image 1: Fer (5D2) Mouse mAb
Western blot analysis of extracts from NIH/3T3, A549 and SK-MEL-5 cells using Fer (5D2) Mouse mAb.

To Purchase # 4268

Cat. # Size Qty. Price Ships
4268T 20 µl
$152 Jan 22
4268S 100 µl
$336 Jan 22

Supporting Data

REACTIVITY H M
SENSITIVITY Endogenous
MW (kDa) 95
Source/Isotype Mouse IgG1
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Simple Western™ 1:50 - 1:250
Immunoprecipitation 1:100

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Fer (5D2) Mouse mAb detects endogenous levels of total Fer protein. This antibody does not cross-react with other related proteins.

Species Reactivity:

Human, Mouse

Source / Purification

Monoclonal antibody is produced by immunizing animals with a truncated recombinant Fer protein.

Background

Fer is a nonreceptor protein-tyrosine kinase of the Fes/Fps family. Like many other cytoplasmic tyrosine kinases, Fer contains a long amino-terminal domain, a central SH2 domain, and a carboxy-terminal kinase domain. Its amino-terminal domain is responsible for protein oligomerization as well as interaction with cytoskeletal proteins. Fer is ubiquitously expressed in a wide variety of cell and tissue types, and is localized to both cytoplasm and nucleus (1). Tyrosine kinase activity of Fer can be stimulated by growth factors and cytokines (2,3). After activation, Fer can further activate various downstream signaling components including Stat3 (3). Fer plays an important role in regulation of cell movement, oncogenesis and inflammation (4).

Pathways

Explore pathways related to this product.


For Research Use Only. Not For Use In Diagnostic Procedures.
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