Firefly Luciferase (E9W9V) Rabbit mAb #25039

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Supporting Data

REACTIVITY	All
SENSITIVITY	Transfected Only
MW (kDa)	62
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting
IF-Immunofluorescence

Species Cross-Reactivity Key:

All-All Species Expected

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunofluorescence (Immunocytochemistry)	1:50 - 1:200

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Firefly Luciferase (E9W9V) Rabbit mAb recognizes exogenously expressed proteins containing firefly luciferase (P. pyralis). This antibody may also recognize a non-specific band of unknown origin at 70 kDa in rat cell lines.

Species Reactivity:

All Species Expected

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln230 of firefly luciferase (P. pyralis) protein.

Background

Luciferases are a group of enzymes that emit visible light in the presence of oxygen and luciferin. Firefly luciferase (FLuc) is a 62 kDa oxygenase isolated from the firefly, P. pyralis, that produces a yellow-green light with a peak emission wavelength of 550-570 nm (1). FLuc is widely used as a bioluminescent reporter, emitting light through an ATP-dependent reaction involving oxygen, D-luciferin, and a metal cation, typically magnesium (Mg²⁺) (2). FLuc is often genetically engineered into luciferase-containing plasmids as a reporter that can be measured by detection of firefly luciferase (P. pyralis) protein or its enzymatic activity (2,3).

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For Research Use Only. Not For Use In Diagnostic Procedures.

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