Render Target: STATIC
Render Timestamp: 2024-12-26T11:55:38.095Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-09-30 01:58:36.770
Product last modified at: 2024-12-17T20:45:08.993Z
Cell Signaling Technology Logo
1% for the planet logo
PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

FIS1 (E3K9O) Rabbit mAb #32525

Filter:
  • WB
  • IP
  • IF

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 17
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50
    Immunofluorescence (Frozen) 1:200 - 1:800
    Immunofluorescence (Immunocytochemistry) 1:200 - 1:800

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    FIS1 (E3K9O) Rabbit mAb recognizes endogenous levels of total FIS1 protein.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human FIS1 protein.

    Background

    Changes in mitochondrial dynamics regulated by environmental cues affect mitochondrial size and shape and have been shown to dramatically impact mitochondrial metabolism, apoptosis, and autophagy (1). Mitochondrial fusion (elongation) and fission (fragmentation) are dynamically controlled by several interacting proteins. Mitochondrial fission is controlled by the GTPase DRP1 which is recruited to the mitochondria by FIS1 and MFF. FIS1 is localized to the mitochondrial outer membrane and overexpression induces fission and leads to apoptosis (2-4). Decreased expression of FIS1 leads to mitochondrial elongation and triggers cellular senescence (5). Elevated expression of FIS1 can also promote autophagy (6). Studies also found that FIS1 interacts with the autophagy initiation protein STX17 and that depletion of FIS1 leads to an increase in mitochondrial localized STX17 and mitophagy (7). Expression of FIS1 has been associated with neurodegeneration and disease, including cancer (8).
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    XP is a registered trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.