FITM2 (E5O8W) Rabbit mAb #62622
- WB
- IP
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 22 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:10 - 1:50 |
| Immunoprecipitation | 1:100 |
Storage
Protocol
Specificity / Sensitivity
FITM2 (E5O8W) Rabbit mAb recognizes endogenous levels of total FITM2 protein. This antibody detects a 60 kDa band of unknown origin in some cell lines.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln135 of human FITM2 protein.
Background
FITM2 (FIT2, C20orf142, acyl-coenzyme A diphosphatase FITM2, fat storage-inducing transmembrane protein 2) is a member of the FIT family of proteins that localize to the endoplasmic reticulum (ER) membrane (1-6). FITM2 is highly expressed by adipocytes and is essential for ER lipid metabolism and lipid droplet formation (1-6). FITM2 maintains ER homeostasis by hydrolyzing fatty acyl–CoA to acyl 4′-phosphopantetheine (6).
- Kadereit, B. et al. (2008) Proc Natl Acad Sci USA 105, 94-9.
- Bai, S.W. et al. (2011) BMC Biol 9, 54.
- Miranda, D.A. et al. (2014) J Biol Chem 289, 9560-72.
- Goh, V.J. et al. (2015) J Biol Chem 290, 25686-99.
- Becuwe, M. et al. (2020) J Cell Biol 219, e202006111. doi: 10.1083/jcb.202006111.
- Bond, L.M. et al. (2023) J Biol Chem 299, 103022.
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