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FKBP1A/FKBP12 Antibody #55104

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  • WB
Western blot analysis of extracts from various cell lines using FKPB1A/FKBP12 Antibody (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

To Purchase # 55104

Cat. # Size Qty. Price
55104S 100 µl
$306

Product Specifications

REACTIVITY H M R
SENSITIVITY Endogenous
MW (kDa) 12
SOURCE Rabbit
Application Key:
  • WB-Western Blotting 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Simple Western™ 1:10

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

FKBP1A/FKBP12 Antibody recognizes endogenous levels of total FKBP1A/FKBP12 protein.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe49 of human FKBP1A/FKBP12 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

FKBP12 (FKBP1A) is a prototypical member of the FKBP (FK506 binding protein) family of immunophilins, so named because of the ability of FKBP12 to bind to the immunosuppressive drug FK506 (tacrolimus) (1). The protein is the smallest member in the family and contains only one peptidylprolyl isomerase (PPIase) core domain (FK domain) responsible for its PPIase activity and its binding to FK506 and other compounds (e.g., rapamycin). When bound to FK506 or rapamycin, the protein:drug complex further binds and inhibits two important signaling molecules: calcineurin, a key enzyme in T cell activation, and the metabolic enzyme mTOR. The inhibition of these pathways has been functionally linked to immunosuppression (1,2). Through its binding properties, FKBP12 also plays regulatory roles in other pathways. For example, the ryanodine receptor (RyR), a type of Ca2+ release channel, exhibits leakiness in the absence of FKBP12 binding, leading to reduced muscle contractility (3). FKBP12 can also bind TGFBR1 and prevent ligand independent activation (4). The protein also mediates MDM2 degradation by binding and disrupting MDM2/MDM4 association, thereby inducing MDM2 self-ubiquitination and enhancing the sensitivity of cells to chemotherapy induced cellular apoptosis (5).

Pathways

Explore pathways related to this product.


Product #55104
Citations: 33

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Showing 8 Images from 33 Citations
Showing 33 Citations
Journal:
bioRxiv
Author:
Gemma Martínez-García, et al.
Year:
2025
Applications:
Unspecified
Reactivity:
Unspecified
Applications:
Unspecified
Reactivity:
Unspecified
Journal:
J Virol
Author:
Cuiling Zhang, et al.
Year:
2024
Applications:
Unspecified
Reactivity:
Unspecified
Journal:
Cancer Cell
Author:
James D Phelan, et al.
Year:
2024
Applications:
Unspecified
Reactivity:
Unspecified
Journal:
bioRxiv
Author:
Devanarayanan Siva Sankar, et al.
Year:
2023
Applications:
Western Blotting (WB)
Reactivity:
Unspecified
For Research Use Only. Not for Use in Diagnostic Procedures.
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