Render Target: STATIC
Render Timestamp: 2024-11-21T14:13:24.733Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-09-30 01:57:36.345
Product last modified at: 2024-09-30T08:02:05.801Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

FLASH/CASP8AP2 (D3T8Q) Rabbit mAb #43339

Filter:
  • WB

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 223
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    FLASH/CASP8AP2 (D3T8Q) Rabbit mAb recognizes endogenous levels of total FLASH/CASP8AP2 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant protein specific to residues near the amino terminus of human FLASH/CASP8AP2 protein.

    Background

    FLICE associated huge protein (FLASH), also termed Caspase 8 associated protein 2 (CASP8AP2), is a large multifunctional protein that has been shown to be involved in multiple processes, including apoptosis, cell cycle progression, and transcriptional regulation (1-3). It was originally identified as a component of the FAS-FADD-Caspase 8 complex with a role in the activation of caspase-8 during Fas-mediated apoptosis (1). However, studies suggest additional functions for FLASH/CASP8AP2 in the nucleus within Cajal and PML bodies (3-5). One of the functions identified was its ability to regulate histone gene processing and S-phase progression (3,6). FLASH/CASP8AP2 has also been found to regulate the activity of transcription factors, including the glucocorticoid receptor and c-Myb (2,7). Furthermore, FLASH/CASP8AP2 was found to stabilize the expression of ZEB1, a transcriptional repressor of genes associated with epithelial-to-mesenchymal transition (EMT), a process associated with cancer metastasis (8). Aberrant expression as well as genetic mutations in the FLASH/CASP8AP2 gene have been described in several cancers and it has been suggested to be a prognostic indicator in acute lymphoblastic leukemia (9-14).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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