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XML generation date: 2024-08-01 15:29:08.788
Product last modified at: 2025-01-01T09:02:56.956Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

FnCpf1/Cas12a (Strain U112) Antibody #59201

Filter:
  • WB
Western Blotting Image 1: FnCpf1/Cas12a (Strain <i>U112</i>) Antibody
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing FnCpf1/Cas12a (Strain U112), AsCpf1/Cas12a (Strain BV3L6), Cas9 (S. pyogenes), or Cas9 (S. aureus) (+), using FnCpf1/Cas12a (Strain U112) Antibody (upper), Myc-Tag (71D10) Rabbit mAb #2278 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower).

To Purchase # 59201

Cat. # Size Qty. Price Ships
59201S 100 µl
$306

Supporting Data

REACTIVITY All
SENSITIVITY Transfected Only
MW (kDa) 152
SOURCE Rabbit
Application Key:
  • WB-Western Blotting 
Species Cross-Reactivity Key:
  • All-All Species Expected 

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

FnCpf1/Cas12a (Strain U112) Antibody recognizes transfected levels of total FnCpf1/Cas12a (Strain U112) protein. This antibody does not cross-react with Cas9 (S. pyogenes), Cas9 (S. aureus), and AsCpf1/Cas12a (Strain BV3L6) proteins.

Species Reactivity:

All Species Expected

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala216 of Cpf1/Cas12a from Francisella tularensis subsp. novicida (Strain U112). Antibodies are purified by protein A and peptide affinity chromatography.

Background

CRISPR-Cas (clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins) are RNA-guided nuclease effectors that are utilized for precise genome editing in mammalian systems (1). Cpf1/Cas12a (CRISPR from Prevotella and Francisella) proteins are members of the Class 2 CRISPR system (2). Class 2 CRISPR systems, such as the well characterized Cas9, rely on single-component effector proteins to mediate DNA interference (3). Cpf1/Cas12a endonucleases, compared to Cas9 systems, have several unique features that increase the utility of CRISPR-based genome editing techniques: 1) Cpf1/Cas12a-mediated cleavage relies on a single and short CRISPR RNA (crRNA) without the requirement of a trans-activating crRNA (tracrRNA), 2) Cpf1/Cas12a utilizes T-Rich protospacer-adjacent motif (PAM) sequences rather than a G-Rich PAM, and 3) Cpf1/Cas12a generates a staggered, rather than a blunt-ended, DNA double-stranded break (2). These features broaden the utility of using CRISPR-Cas systems for specific gene regulation and therapeutic applications. Several Cpf1/Cas12a bacterial orthologs have been characterized for CRISPR-mediated mammalian genome editing (2,4).~FnCpf1 (Strain U112)/Cas12a is a Cpf1/Cas12a enzyme derived from Francisella novicida U112 (5).
For Research Use Only. Not For Use In Diagnostic Procedures.
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