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Product last modified at: 2024-06-27T13:37:02.334Z
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PDP - Template Name: Antibody Sampler Kit
PDP - Template ID: *******4a3ef3a
  1. Products /
  2. Primary Antibodies /
  3. Fragile X/FMRP Signaling Pathway Antibody Sampler Kit

Fragile X/FMRP Signaling Pathway Antibody Sampler Kit #48267

    Product Information

    Kit Usage Information

    Protocols

    Product Description

    The Fragile X/FMRP Signaling Pathway Antibody Sampler Kit provides an economical means of detecting signaling components of the Fragile X/FMRP signaling pathway. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.

    Specificity / Sensitivity

    Each antibody in the Fragile X/FMRP Signaling Pathway Antibody Sampler Kit detects endogenous levels of its target protein. Phospho-eEF2 (Thr56) Antibody detects endogenous levels of eEF2 only when phosphorylated at Thr56. It does not recognize eEF2 phosphorylated at other sites.

    Source / Purification

    Monoclonal antibodies are produced by immunizing rabbits with synthetic peptides corresponding to Gly552 of human FMRP protein, Leu1105 of human mGluR1 protein, Gly574 of human FXR1 protein, and Gly477 of human FXR2 protein. mGluR5 (D6E7B) Rabbit mAb is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human mGluR5 protein. eEF2 Antibody and CYFIP1 Antibody are produced by immunizing animals with a synthetic peptide corresponding to residues at the amino-terminus of human eEF2 and residues surrounding Pro527 of human CYFIP1 protein, respectively. Phospho-eEF2 (Thr56) Antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr56 of human eEF2. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Fragile X syndrome, a frequent cause of inherited mental retardation, often results from expansion of the CGG trinucleotide repeat in the gene that encodes the fragile X mental retardation protein (FMRP, [1]). FMRP (also known as FMR1) and its two autosomal homologs (FXR1 and FXR2) all bind RNA and play a role in the pathogenesis of fragile X syndrome (1-3). Each of these related proteins can associate with one another as well as form homodimers and complexes with other RNA-binding proteins like cytoplasmic FMRP interacting protein 1 (CYFIP1, [3,4]). FMRP, FXR1, FXR2, and CYFIP1 have been implicated in the translational regulation of mRNAs (5,6). Importantly, this complex of proteins may be dynamically regulated to drive protein synthesis-dependent forms of synaptic plasticity in response to specific activity. That is, activation of metabotropic glutamate receptors, including mGluR1 and mGlur5, can regulate FMRP-dependent forms of translation via post-translational modification of eukaryotic elongation factor 2 (eEF2) to locally control dynamic translation of important synaptic proteins, which, subsequently, alter synaptic function (7-9).   

    Database Links

    UniProt ID: P51114 , P41594 , Q06787 , P13639 , Q13255 , Q7L576 , P51116


    Entrez-Gene Id: 8087 , 2915 , 2332 , 1938 , 2911 , 23191 , 9513


    Limited Uses

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    Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

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