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Product last modified at: 2025-01-01T09:02:05.137Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

FSTL1 (E4Y1F) Rabbit mAb #77561

Filter:
  • WB
Western Blotting Image 1: FSTL1 (E4Y1F) Rabbit mAb
Western blot analysis of extracts from various cell lines using FSTL1 (E4Y1F) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Negative expression of FSTL1 protein in Hep G2 and Ramos cells is consistent with the predicted expression pattern.

To Purchase # 77561

Cat. # Size Qty. Price Ships
77561T 20 µl
$156
77561S 100 µl
$371

Supporting Data

REACTIVITY H M R
SENSITIVITY Endogenous
MW (kDa) 40
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

FSTL1 (E4Y1F) Rabbit mAb recognizes endogenous levels of total FSTL1 protein.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human FSTL1 protein.

Background

Follistatin-related protein 1 (FSTL1, TSC-36, or FRP) is a secreted glycoprotein that is a member of the SPARC family (1). FSTL1 modulates multiple biological processes through various signaling pathways. FSTL1 acts as an antagonist of BMP4 to regulate the TGF-β/BMP/Smad signaling cascade (2), which is required for lung, smooth muscle, and skeletal development (2-4). As a ligand of the receptor DIP2A, FSTL1 activates downstream Akt/eNOS signaling (5), which protects the heart during injury and promotes tissue regeneration during ischemia stress and cardiovascular disease progression (6,7). FSTL1 is involved in the activation of several additional signaling cascades, such as NF-κB, Erk, AMPK, and Wnt/β-Catenin pathways (8,9). By mediating these signaling pathways, FSTL1 contributes to immune inflammatory response (10), cancer progression, cancer-associated immune infiltration, and metastasis (11-13).
For Research Use Only. Not For Use In Diagnostic Procedures.
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