Render Target: STATIC
Render Timestamp: 2024-11-28T11:27:08.673Z
Commit: d79925545b26f8827f92d145dadc6f0527debdb1
XML generation date: 2024-08-01 15:28:24.813
Product last modified at: 2024-10-25T20:30:10.975Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

FTSJ3 Antibody #71873

Filter:
  • WB

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 120
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    FTSJ3 Antibody recognizes endogenous levels of total FTSJ3 protein.

    Species Reactivity:

    Human

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ile651 of human FTSJ3 protein. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    FTSJ3 is an RNA 2’-O-methyltransferase originally discovered through its role in processing of 34S pre-rRNA (1). In addition to contributing to RNA stabilization, 2’-O-methylation at the 5’-cap enables the innate immune system to distinguish self from non-self mRNA (2). While some viruses encode their own 2’-O-methyltransferase enabling them to avoid detection by the innate immune system, HIV does not (3,4). However, it was recently demonstrated that FTSJ3 is recruited to HIV RNA resulting in 2’-O-methylation of the HIV RNA genome and evasion of immune detection (5). Cells deficient in FTSJ3 produced virus with less 2’-O-methylation and upregulated type I interferon expression leading to reduced HIV expression and replication (5).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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