Name: FTSJ3 Antibody
Price: 302.00 USD
Availability: InStock

Filter:

WB

Supporting Data

REACTIVITY	H
SENSITIVITY	Endogenous
MW (kDa)	120
SOURCE	Rabbit

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

H-Human

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

FTSJ3 Antibody recognizes endogenous levels of total FTSJ3 protein.

Species Reactivity:

Human

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ile651 of human FTSJ3 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

FTSJ3 is an RNA 2’-O-methyltransferase originally discovered through its role in processing of 34S pre-rRNA (1). In addition to contributing to RNA stabilization, 2’-O-methylation at the 5’-cap enables the innate immune system to distinguish self from non-self mRNA (2). While some viruses encode their own 2’-O-methyltransferase enabling them to avoid detection by the innate immune system, HIV does not (3,4). However, it was recently demonstrated that FTSJ3 is recruited to HIV RNA resulting in 2’-O-methylation of the HIV RNA genome and evasion of immune detection (5). Cells deficient in FTSJ3 produced virus with less 2’-O-methylation and upregulated type I interferon expression leading to reduced HIV expression and replication (5).

Database Links

UniProt ID: Q8IY81

Entrez-Gene Id: 117246

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.

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FTSJ3 Antibody #71873