Name: FTSJ3 (E6T9J) Rabbit mAb
Price: 327.00 USD
Availability: InStock

Filter:

WB

Supporting Data

REACTIVITY	H M
SENSITIVITY	Endogenous
MW (kDa)	120
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

H-Human
M-Mouse

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

FTSJ3 (E6T9J) Rabbit mAb recognizes endogenous levels of total FTSJ3 protein.

Species Reactivity:

Human, Mouse

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ile651 of human FTSJ3 protein.

Background

FTSJ3 is an RNA 2’-O-methyltransferase originally discovered through its role in processing of 34S pre-rRNA (1). In addition to contributing to RNA stabilization, 2’-O-methylation at the 5’-cap enables the innate immune system to distinguish self from non-self mRNA (2). While some viruses encode their own 2’-O-methyltransferase enabling them to avoid detection by the innate immune system, HIV does not (3,4). However, it was recently demonstrated that FTSJ3 is recruited to HIV RNA resulting in 2’-O-methylation of the HIV RNA genome and evasion of immune detection (5). Cells deficient in FTSJ3 produced virus with less 2’-O-methylation and upregulated type I interferon expression leading to reduced HIV expression and replication (5).

Database Links

UniProt ID: Q8IY81

Entrez-Gene Id: 117246

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.

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FTSJ3 (E6T9J) Rabbit mAb #50607