Render Target: STATIC
Render Timestamp: 2024-11-21T13:51:18.900Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-11-18 16:03:16.446
Product last modified at: 2024-11-19T09:00:17.631Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

G3BP2 (F3Y7Z) Mouse mAb #86863

Filter:
  • WB
  • IF

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 60, 70
    Source/Isotype Mouse IgG1 kappa
    Application Key:
    • WB-Western Blotting 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunofluorescence (Immunocytochemistry) 1:100 - 1:400

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    G3BP2 (F3Y7Z) Mouse mAb recognizes endogenous levels of total G3BP2 protein. This antibody does not cross-react with G3BP1 protein. In rodent cells/tissue, this antibody may cross-react with a band of unknown origin around 48 kDa.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human G3BP2 protein.

    Background

    Stress granules (SGs) are cytoplasmic mRNA-protein condensates formed in response to cellular stressors, such as oxidative stress, ultraviolet radiation, and viral infection (1). The Ras GTPase-activating protein-binding proteins (G3BPs), consisting of G3BP1 and G3BP2, are key nucleating factors essential for SG formation (2). As their name suggests, G3BPs were originally identified as proteins that interact with the SH3 domain of Ras GTPase-activating protein (RasGAP) (3). However, a recent study argues that G3BP1 may not be a genuine RasGAP-binding partner, suggesting that further studies are necessary to validate this function (4). Rather, G3BPs are well known to be involved in mRNA decay and inhibition of translation initiation (5,6). The SARS-CoV-2 virus has also been reported to target G3BPs via interaction between the viral N protein and the N-terminal domain of G3BP. This binding prevents G3BPs from interacting with other SG-associated proteins, therefore suppressing SG assembly and promoting viral replication (7). G3BP2, in particular, has also been reported to play a role in the initiation of breast tumor formation and affect the expression of PD-L1 in breast and glioblastoma cells under stress conditions (8,9).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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