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R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

GADD34 (E3S6N) Rabbit mAb #41222

Filter:
  • WB
Western Blotting Image 1: GADD34 (E3S6N) Rabbit mAb
Western blot analysis of extracts from SK-N-AS cells, vehicle-treated (-) or treated with Thapsigargin #12758 (1 μM, 8 hr; +), using GADD34 (E3S6N) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). GADD34 is induced by thapsigargin treatment as expected.

To Purchase # 41222

Cat. # Size Qty. Price
41222T 20 µl
$153
41222S 100 µl
$339

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 110
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
Species Cross-Reactivity Key:
  • H-Human 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

GADD34 (E3S6N) Rabbit mAb recognizes endogenous levels of total GADD34 protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala69 of human GADD34 protein.

Background

The growth arrest and DNA damage-inducible protein, GADD34, is essential for cell stress responses. It is induced by stresses such as DNA damage and endoplasmic reticulum (ER) stress. ER stress stimulates the unfolded protein response (UPR), increasing phosphorylation of Ser51 in the α subunit of eukaryotic translation initiation factor 2 (eIF2α). This change represses translation. Phosphorylation of eIF2α also increases GADD34 expression. GADD34 binds to type 1 protein phosphatase 1 (PP1), a serine/threonine phosphatase, and promotes PP1 to dephosphorylate eIF2α (1,2). GADD34 expression is correlated with eIF2α dephosphorylation late in the stress response, suggesting a critical role of GADD34 in a negative feedback loop to reduce stress response signaling and shift cells from protein synthesis inhibition to stress-induced gene expression (3).

Pathways

Explore pathways related to this product.


For Research Use Only. Not For Use In Diagnostic Procedures.
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