GAK (E7W7Z) Rabbit mAb #51509
- WB
- IP
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 175 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Protocol
Specificity / Sensitivity
GAK (E7W7Z) Rabbit mAb recognizes endogenous levels of total GAK protein. A band of unknown origin is detected at ~45 kDa and at ~20kDa.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro758 of human GAK protein.
Background
Cyclin-G-associated kinase (GAK) is the ubiquitous form of the neuronal-specific protein auxilin 1 (1) and is an essential cofactor for Hsc70-dependent uncoating of clathrin-coated vesicles in non-neuronal cells (2). The recruitment of GAK to clathrin-coated pits during endocytosis is dependent on its PTEN-like domain, which has been shown to bind to phospholipids (3). It has been postulated that the JAK inhibitor baricitinib may impair entry of the SARS-CoV-2 virus by inhibiting AAK1- and GAK-mediated endocytosis via ACE2 (4). In the nucleus, GAK forms complexes with cyclin G1 and other nuclear proteins (5) and plays an essential role in proper mitotic progression by maintaining centrosome structure (6). GAK is also required for the regulation of basal, PRKN-independent mitophagy by modifying the mitochondrial network and lysosomal morphology that compromise efficient transport of mitochondria for degradation (7,8).
- Kanaoka, Y. et al. (1997) FEBS Lett 402, 73-80.
- Greener, T. et al. (2000) J Biol Chem 275, 1365-70.
- Lee, D.W. et al. (2006) J Cell Sci 119, 3502-12.
- Stebbing, J. et al. (2020) Lancet Infect Dis 20, 400-402.
- Sato, J. et al. (2009) Genes Cells 14, 627-41.
- Shimizu, H. et al. (2009) J Cell Sci 122, 3145-52.
- Munson, M.J. et al. (2021) Nat Commun 12, 6101.
- Munson, M.J. et al. (2022) Autophagy 18, 467-469.
Limited Uses
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