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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

Galectin-3/LGALS3 Antibody #12733

Filter:
  • WB
Western Blotting Image 1: Galectin-3/LGALS3 Antibody
Western blot analysis of extracts from various cell lines using Galectin-3/LGALS3 Antibody.

To Purchase # 12733

Cat. # Size Qty. Price Ships
12733S 100 µl
$306

Supporting Data

REACTIVITY H M R
SENSITIVITY Endogenous
MW (kDa) 28
SOURCE Rabbit
Application Key:
  • WB-Western Blotting 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Galectin-3/LGALS3 antibody recognizes endogenous levels of total galectin-3/LGALS3 protein.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human galectin-3/LGALS3 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Galectins are a family of β-galactose binding proteins that are characterized by an affinity for poly-N-acetyllactosamine-enriched glycoconjugates and a carbohydrate-binding site (1,2). Members of the galectin family have been implicated in a variety of biological functions, including cell adhesion (3), growth regulation (4), cytokine production (5), T cell apoptosis (6), and immune responses (7).

Galectin-3/LGALS3 is involved in several diverse biological functions. Galectin-3/LGALS3 binds IgE (8). Galectin-3/LGALS3 is an unusual protein in that it can be found both extracellularly and intracellularly. Intracellularly, Galectin-3/LGALS3 can localize to the cytoplasm, nucleus, or both, depending on cell type and experimental conditions. Nuclear Galectin-3/LGALS3 has been identified as a pre-mRNA splicing factor (9). Galectin-3/LGALS3 production has been shown to increase during inflammation and in obesity, and the protein itself can have an inflammatory effect under certain conditions (10). Galectin-3/LGALS3 forms a complex with α3, β1 integrin to act as a surface receptor on endothelial cells for the NG2 proteoglycan, which triggers cell motility and angiogenesis (11). In addition to these functions, Galectin-3/LGALS3 is also a required factor for the terminal differentiation of epithelial cells (12).
For Research Use Only. Not For Use In Diagnostic Procedures.
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