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XML generation date: 2025-03-07 13:12:03.166
Product last modified at: 2025-03-28T17:00:09.552Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

GAP43 (D9C8) Rabbit mAb #8945

Filter:
  • WB
  • IP
  • IF
Western Blotting Image 1: GAP43 (D9C8) Rabbit mAb
Western blot analysis of extracts from mouse neonatal brain and rat fetal brain using GAP43 (D9C8) Rabbit mAb.

To Purchase # 8945

Cat. # Size Qty. Price
8945T 20 µl
$153
8945S 100 µl
$339

Supporting Data

REACTIVITY H M R
SENSITIVITY Endogenous
MW (kDa) 38, 43
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
  • IF-Immunofluorescence 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:50
Immunofluorescence (Frozen) 1:200 - 1:400

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #42786.

Protocol

Specificity / Sensitivity

GAP43 (D9C8) Rabbit mAb recognizes endogenous levels of total GAP43 protein. Low levels of non-specific nuclear labeling may be observed by immunocytochemistry (ICC).

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys30 of human GAP43 protein.

Background

GAP43 is a nervous system specific, growth-associated protein enriched in growth cones and areas of high plasticity (1). Phosphorylation of GAP43 at Ser41 by PKC is regulated by intracellular Ca2+ and affects the ability of GAP43 to bind calmodulin (2,3). GAP43 is integral to growth cone formation, neurite outgrowth, and the development of a functional cerebral cortex (4,5). Aberrant GAP43 expression can be seen in patients diagnosed with schizophrenia and Alzheimer's Disease (6,7).
For Research Use Only. Not For Use In Diagnostic Procedures.
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