GAP43 (E1U1D) Mouse mAb (BSA and Azide Free) #47235
- WB
- IF
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 38, 43 |
| Source/Isotype | Mouse IgG1 kappa |
Application Key:
- WB-Western Blotting
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
Formulation
Storage
Specificity / Sensitivity
GAP43 (E1U1D) Mouse mAb (BSA and Azide Free) recognizes endogenous levels of total GAP43 protein. This antibody detects a non-specific band of unknown origin at 110 kDa.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of mouse GAP43 protein.
Background
GAP43 is a nervous system specific, growth-associated protein enriched in growth cones and areas of high plasticity (1). Phosphorylation of GAP43 at Ser41 by PKC is regulated by intracellular Ca2+ and affects the ability of GAP43 to bind calmodulin (2,3). GAP43 is integral to growth cone formation, neurite outgrowth, and the development of a functional cerebral cortex (4,5). Aberrant GAP43 expression can be seen in patients diagnosed with schizophrenia and Alzheimer's Disease (6,7).
- Biewenga, J.E. et al. (1996) Acta Biochim Pol 43, 327-38.
- Dekker, L.V. and Parker, P.J. (1997) J Biol Chem 272, 12747-53.
- Chapman, E.R. et al. (1991) J Biol Chem 266, 207-13.
- Aigner, L. and Caroni, P. (1993) J Cell Biol 123, 417-29.
- Shen, Y. et al. (2008) Cerebellum 7, 451-66.
- Sower, A.C. et al. (1995) Mol Chem Neuropathol 24, 1-11.
- de la Monte, S.M. et al. (1995) Am J Pathol 147, 934-46.
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