Render Target: STATIC
Render Timestamp: 2024-11-21T13:09:42.053Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-08-30 10:36:59.726
Product last modified at: 2024-08-31T07:01:30.743Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

GAP43 (E1U1D) Mouse mAb (BSA and Azide Free) #47235

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  • IF

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 38, 43
    Source/Isotype Mouse IgG1 kappa
    Application Key:
    • WB-Western Blotting 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    This product is the carrier free version of product #30817. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

    This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

    BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.

    Formulation

    Supplied in 1X PBS (10 mM Na2HPO4, 3 mM KCl, 2 mM KH2PO4, and 140 mM NaCl (pH 7.8)). BSA and Azide Free.

    For standard formulation of this product see product #30817

    Storage

    Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

    Specificity / Sensitivity

    GAP43 (E1U1D) Mouse mAb (BSA and Azide Free) recognizes endogenous levels of total GAP43 protein. This antibody detects a non-specific band of unknown origin at 110 kDa.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of mouse GAP43 protein.

    Background

    GAP43 is a nervous system specific, growth-associated protein enriched in growth cones and areas of high plasticity (1). Phosphorylation of GAP43 at Ser41 by PKC is regulated by intracellular Ca2+ and affects the ability of GAP43 to bind calmodulin (2,3). GAP43 is integral to growth cone formation, neurite outgrowth, and the development of a functional cerebral cortex (4,5). Aberrant GAP43 expression can be seen in patients diagnosed with schizophrenia and Alzheimer's Disease (6,7).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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