Render Target: STATIC
Render Timestamp: 2024-11-22T12:09:54.557Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-08-01 15:28:23.162
Product last modified at: 2024-09-26T11:45:38.331Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

GAT3 Antibody #25438

Filter:
  • WB

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 70, 140
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    GAT3 Antibody recognizes endogenous levels of total GAT3 protein.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro604 of human GAT3 protein. Antibodies are purified by peptide affinity chromatography.

    Background

    The solute carrier 6 (SLC6) gene, also known as the neurotransmitter–sodium-symporter family or Na+/Cl- -dependent transporters, encodes for proteins that regulate neurotransmitter (NTT) transport, including monoamine transmitters serotonin, dopamine, and norepinephrine (SERT), GABA transmitters (GAT1, GAT2, GAT3, and BGT1), and glycine transmitters (GLYT1 and GLYT2) (1). The sodium- and chloride-dependent GABA transporter 3 (GAT3) is encoded by the solute carrier family 6 member 11 (SLC6A11) gene (2). GABA is the principal inhibitory neurotransmitter in the central nervous system and maintains the inhibitory tone that balances out neuronal excitation. This balance between neuronal excitation and inhibition is tightly regulated, in part by the GAT family of GABA transporters. These transporters terminate GABA inhibition by mediating cellular reuptake of GABA at the synaptic cleft. The human genome encodes four GABA transports (GAT-1-4).  GAT-1 is found predominantly in the axon terminals of neurons whereas GAT-3 is expressed in astrocytes (3). Dysfunction of GABA transporters may contribute to diseases such as epilepsy as well as neurodegenerative diseases such as Alzheimer’s and Parkinson’s disease (4-6).
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