GATA-2 (E8B5C) Rabbit mAb (BSA and Azide Free) #67509

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Supporting Data

REACTIVITY	H
SENSITIVITY	Endogenous
MW (kDa)	51
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting
IHC-Immunohistochemistry
IF-Immunofluorescence

Species Cross-Reactivity Key:

H-Human

Product Information

Product Usage Information

This product is the carrier free version of product #72445. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.

Formulation

Supplied in 1X PBS (10 mM Na₂HPO₄, 3 mM KCl, 2 mM KH₂PO₄, and 140 mM NaCl (pH 7.8)). BSA and Azide Free.

For standard formulation of this product see product #72445

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

GATA-2 (E8B5C) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total GATA-2 protein. Non-specific staining was observed in squamous epithelium by immunohistochemistry.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly132 of human GATA-2 protein.

Background

The zinc finger transcription factor GATA-2 is widely expressed and plays an essential role in many developmental processes (1). Studies on GATA-2 knockout mice indicate that this protein is required in hematopoiesis (2). GATA-2 also inhibits the differentiation of white (3) and brown adipocytes (4) and has been shown to suppress the proliferation of neuronal progenitor cells (5).

Database Links

UniProt ID: P23769

Entrez-Gene Id: 2624

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.

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