Render Target: STATIC
Render Timestamp: 2024-12-04T10:38:15.540Z
Commit: cd2fae6ca3f811b1ddb1df24ac291ed56d5d501b
XML generation date: 2024-04-05 20:24:56.418
Product last modified at: 2024-05-30T07:12:15.245Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

GATA-4 Antibody #14353

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Inquiry Info. # 14353

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    Supporting Data

    REACTIVITY H M
    SENSITIVITY Endogenous
    MW (kDa) 55
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    GATA-4 Antibody recognizes endogenous levels of total GATA-4 protein.

    Species Reactivity:

    Human, Mouse

    The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

    Species predicted to react based on 100% sequence homology:

    Rat

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human GATA-4 protein. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    GATA proteins comprise a group of transcription factors that are related by the presence of conserved zinc finger DNA binding domains, which bind directly to the nucleotide sequence core element GATA (1-3). There are six vertebrate GATA proteins, designated GATA-1 to GATA-6 (3).
    GATA-4 is crucial for cardiomyocyte differentiation (4), and not surprisingly, mutations in the GATA-4 gene are implicated in many cardiac diseases, such as tetralogy of Fallot (5), familial and sporadic dilated cardiomyopathy (6,7), and atrial septal defect (8). GATA-4 and GATA-6 together maintain intestinal epithelial structure by regulating enterocyte gene expression (9). They also have overlapping roles in steroidogenesis and genital ridge formation during gonadal development (10). GATA-4 is also a required factor for bone mineralization in osteoblast progenitor cells, where it suppresses TGFβ and activates BMP signaling (11). Research studies have uncovered crosstalk among the transcription factors GATA-4, GATA-6, and KLF5 that promotes gastric cancer development (12). Additional studies describe the role of histone H3K9 hyperacetylation on the GATA-4 promoter in fetal mice that have been exposed to ethanol (13).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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