Render Target: STATIC
Render Timestamp: 2024-11-22T11:35:46.542Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-11-19 23:03:08.869
Product last modified at: 2024-11-20T09:00:11.128Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

GCH1 (F8T4M) Rabbit mAb #36788

Filter:
  • WB
  • IF

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 28
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunofluorescence (Immunocytochemistry) 1:1600 - 1:3200

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    GCH1 (F8T4M) Rabbit mAb recognizes endogenous levels of total GCH1 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly52 of human GCH1 protein.

    Background

    GTP cyclohydrolase 1 (GCH1) catalyzes the rate-limiting step of tetrahydrobiopterin (BH4) biosynthesis. Studies show that BH4 functions as a radical-trapping antioxidant and protects cells against ferroptosis independently of GPX4. In addition, dihydrofolate reductase (DHFR) is required to maintain BH4 levels by regenerating the compound (1,2). Furthermore, deficiency of spliceosomal GTPase Eftud2 in mouse cerebellar Purkinje cells (PCs) causes severe ferroptosis, PC degeneration, dyskinesia, and cerebellar atrophy. Ablation of Eftud2 in PCs downregulates Gch1 expression through mis-splicing of Atf4, activating ferroptosis (3).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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