Render Target: STATIC
Render Timestamp: 2024-09-26T09:58:19.398Z
Commit: 60a5021c3a47fc24d1656fb463e2c3c41a1ad145
1% for the planet logo
PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

GCSAM/HGAL Antibody #20289

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 25
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    GCSAM/HGAL Antibody recognizes endogenous levels of total GCSAM/HGAL protein.

    Species Reactivity:

    Human

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human GCSAM/HGAL protein. Antibodies are purified by peptide affinity chromatography.

    Background

    Germinal center-associated signaling and motility protein/Human germinal center-associated lymphoma (GCSAM/HGAL) is reported to be a marker for germinal center B cells and germinal center-derived B-cell lymphomas (1). Research studies have shown that GCSAM is an adaptor protein anchored to the plasma membrane via myristoyl and palmitoyl linkages and is phosphorylated on multiple tyrosine residues by Syk and Lyn upon engagement of the B cell antigen receptor (BCR), which promotes its interaction with Grb2 and Syk. Binding of GCSAM to Syk positively regulates BCR signaling by enhancing Syk kinase activity (2-5). In addition to regulating antigen-induced BCR signaling in germinal center B cells, research studies have demonstrated that GCSAM restricts motility of B cells within the germinal center microenvironment through activation of RhoA signaling (6). Through regulation of these two processes, GCSAM has been implicated in the differentiation of germinal center B cells to either plasma cells or memory B cells (7).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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