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Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

GDNF (E5Y9N) Rabbit mAb #96166

Filter:
  • WB
  • IP
Western blot analysis of extracts from 293T cells, mock transfected or transfected with a construct expressing Myc/DDK-tagged full-length human GDNF protein (Myc/DDK-hGDNF), Human GDNF Recombinant Protein #16187, and mouse GDNF recombinant protein using GDNF (E5Y9N) Rabbit mAb (upper), Myc-Tag (71D10) Rabbit mAb #2278 (middle), or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).

To Purchase # 96166

Cat. # Size Qty. Price
96166T 20 µl
$168
96166S 100 µl
$371

Product Specifications

REACTIVITY H M
SENSITIVITY Recombinant and Transfected
MW (kDa) Precursor 28 - Mature 13-16
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

GDNF (E5Y9N) Rabbit mAb recognizes human and mouse recombinant GDNF, and exogenously expressed GDNF protein.

Species Reactivity:

Human, Mouse

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu200 of human GDNF protein.

Background

Glial cell-derived neurotrophic factor (GDNF) plays an important role in the development and maintenance of the central and peripheral nervous system, renal morphogenesis, and spermatogenesis (1). This glycosylated, disulfide-bonded homodimer is a member of the TGF-β superfamily and plays an important role in neuronal survival (2). GDNF and the related GDNF family of ligands (GFLs) neurturin, persephin, and artemin bind to the GDNF family receptor α (GFRα) proteins that signal through the Ret receptor tyrosine kinase (3,4). The effect that GDNF has on degenerating dopamine neurons makes it an important growth factor when studying Parkinson’s disease (PD) (5). GDNF has also been identified as a therapeutic target for amyotrophic lateral sclerosis (ALS), is a suggested biomarker for Alzheimer’s Disease (AD), and has been associated with cancer progression (6-9).
For Research Use Only. Not for Use in Diagnostic Procedures.
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