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Product Name

52508 Geminin (E5Q9S) XP^® Rabbit mAb

Filter:

WB
IP

Inquiry Info. # 5165

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Supporting Data

REACTIVITY	H M Mk
SENSITIVITY	Endogenous
MW (kDa)	25
SOURCE	Rabbit

Application Key:

WB-Western Blotting
IP-Immunoprecipitation

Species Cross-Reactivity Key:

H-Human
M-Mouse
Mk-Monkey

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Geminin Antibody detects endogenous levels of total geminin protein.

Species Reactivity:

Human, Mouse, Monkey

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly47 of human geminin protein. Antibodies are purified using protein A and peptide affinity chromatography.

Background

The initiation of DNA replication in mammalian cells is a highly coordinated process that ensures duplication of the genome only once per cell division cycle. Origins of replication are dispersed throughout the genome and their activities are regulated via the sequential binding of pre-replication and replication factors. The origin recognition complex (ORC) is thought to be bound to chromatin throughout the cell cycle (1,2). The pre-replication complex (pre-RC) forms in late mitosis/early G1 phase beginning with the binding of CDT1 and cdc6 to the origin, which allows binding of the heterohexameric MCM2-7 complex. Once this complex is formed, the origin is “licensed” for initiation of DNA replication. In order to ensure that replication occurs only once per cell cycle, geminin binds to and inhibits CDT1 during the S, G2, and M phases. This prevents the recruitment of the MCM complex to the origins of replication, which blocks the premature reformation of the pre-RC. At the metaphase/anaphase transition, geminin is degraded by the anaphase-promoting complex (APC) allowing for the formation of a new pre-RC (3,4).

Database Links

UniProt ID: O75496

Entrez-Gene Id: 51053

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.

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Geminin Antibody #5165