GIMAP5 Antibody #14108
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 30,32 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Protocol
Specificity / Sensitivity
GIMAP5 Antibody recognizes endogenous levels of total GIMAP5 protein. This antibody also recognizes proteins of unknown origin at 12 kDa and 50 kDa.
Species Reactivity:
Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human GIMAP5 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
GTPase immune-associated proteins (GIMAP), also known as immune-associated nucleotide-binding (IAN) proteins, are evolutionarily conserved GTP-binding proteins involved in lymphocyte development, inflammation, and autoimmune diseases (reviewed in 1,2). Human GTPase IMAP family member 5 (GIMAP5, hIan5) is the homolog of the rat Ian4 protein that is mutated in severe cases of T-cell lymphopenia and insulin-dependent diabetes in Biobreeding diabetes-prone (BB-DP) rats (3,4). GIMAP5 protein is preferentially expressed in CD4- and CD8-positive T-cells as well as B-cell lymphomas (4). Research studies using GIMAP5-deficient mice show that GIMAP5 protein is critical for survival of peripheral T-cells, hematopoietic stem cells, and progenitor cells (5-7). Additional studies indicate that GIMAP5 deficiency leads to a loss of immunological tolerance (8). Polymorphisms in the human GIMAP5 gene are associated with systemic lupus erythematosus and type I diabetes (9-11). Potential mechanisms for GIMAP5 control of cell survival include regulation of Bcl-2 family proteins, mitochondrial integrity, lysosomal function, and calcium regulation (5,7, 11-14).
Limited Uses
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