Render Target: STATIC
Render Timestamp: 2024-11-21T12:59:10.997Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-08-01 15:23:43.722
Product last modified at: 2024-10-30T21:45:07.782Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

GIT-1 Antibody #2919

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H M R Hm Mk B
    SENSITIVITY Endogenous
    MW (kDa) 95
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Hm-Hamster 
    • Mk-Monkey 
    • B-Bovine 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    GIT-1 Antibody recognizes endogenous levels of total GIT-1 protein.

    Species Reactivity:

    Human, Mouse, Rat, Hamster, Monkey, Bovine

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human GIT-1. Antibodies are purified using peptide affinity chromatography.

    Background

    G protein-coupled receptor (GPCR) kinase interacting proteins 1 and 2 (GIT1 and GIT2) are highly conserved, ubiquitous scaffold proteins involved in localized signaling to help regulate focal contact assembly and cytoskeletal dynamics. GIT proteins contain multiple interaction domains that allow interaction with small GTPases (including ARF, Rac, and cdc42), kinases (such as PAK and MEK), the Rho family GEF PIX, and the focal adhesion protein paxillin (reviewed in 1). GIT1 is localized to focal adhesions, cytoplasmic complexes and membrane protrusions, and regulates cell protrusion formation and migration (2). GIT1 has also been implicated in neuronal functions including synapse formation (3) and the pathology of Huntington's disease (HD) (4). HD is a genetic neurodegenerative condition involving a mutation in the htt gene encoding the protein huntingtin. Huntingtin is ubiquitinated and degraded in human HD brains (5). Huntingtin interacts directly with GIT1, causing enhanced proteolysis and indicating that GIT1 distribution and function may contribute to HD pathology (4).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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