Render Target: STATIC
Render Timestamp: 2024-12-26T11:34:53.268Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-09-30 02:00:04.493
Product last modified at: 2024-10-18T17:45:08.543Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. GIT1 (F5R4M) Rabbit mAb
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

GIT1 (F5R4M) Rabbit mAb #92838

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 95
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    GIT1 (F5R4M) Rabbit mAb recognizes endogenous levels of total GIT1 protein.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val421 of human GIT1 protein.

    Background

    G protein-coupled receptor (GPCR) kinase interacting proteins 1 and 2 (GIT1 and GIT2) are highly conserved, ubiquitous scaffold proteins involved in localized signaling to help regulate focal contact assembly and cytoskeletal dynamics. GIT proteins contain multiple interaction domains that allow interaction with small GTPases (including ARF, Rac, and cdc42), kinases (such as PAK and MEK), the Rho family GEF PIX, and the focal adhesion protein paxillin (reviewed in 1). GIT1 is localized to focal adhesions, cytoplasmic complexes and membrane protrusions, and regulates cell protrusion formation and migration (2). GIT1 has also been implicated in neuronal functions including synapse formation (3) and the pathology of Huntington's disease (HD) (4). HD is a genetic neurodegenerative condition involving a mutation in the htt gene encoding the protein huntingtin. Huntingtin is ubiquitinated and degraded in human HD brains (5). Huntingtin interacts directly with GIT1, causing enhanced proteolysis and indicating that GIT1 distribution and function may contribute to HD pathology (4).

    Database Links

    UniProt ID: Q9Y2X7


    Entrez-Gene Id: 28964


    Limited Uses

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    For Research Use Only. Not For Use In Diagnostic Procedures.
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