Glu-Glu Tag (F3Z5J) Rabbit mAb #71756

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Supporting Data

REACTIVITY	All
SENSITIVITY	Transfected Only
MW (kDa)
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting
IP-Immunoprecipitation
IF-Immunofluorescence

Species Cross-Reactivity Key:

All-All Species Expected

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:50
Immunofluorescence (Immunocytochemistry)	1:200 - 1:800

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Glu-Glu Tag (F3Z5J) Rabbit mAb detects recombinant proteins containing the Glu-Glu epitope tag. The antibody recognizes the Glu-Glu sequence fused to either the amino or carboxy terminus of targeted proteins in transfected cells. Glu-Glu Tag (F3Z5J) Rabbit mAb detects exogenously expressed Glu-Glu-tagged proteins in cells expressed under a CMV promoter. Expression under other promoters has not been evaluated. This antibody may detect a band of unknown origin at 30 kDa in some rodent cell lines.

Species Reactivity:

All Species Expected

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic Glu-Glu tag peptide.

Background

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.

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