Glu-Glu Tag (F3Z5J) Rabbit mAb #71756
- WB
- IP
- IF
Supporting Data
| REACTIVITY | All |
| SENSITIVITY | Transfected Only |
| MW (kDa) | |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- All-All Species Expected
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunofluorescence (Immunocytochemistry) | 1:200 - 1:800 |
Storage
Protocol
Specificity / Sensitivity
Glu-Glu Tag (F3Z5J) Rabbit mAb detects recombinant proteins containing the Glu-Glu epitope tag. The antibody recognizes the Glu-Glu sequence fused to either the amino or carboxy terminus of targeted proteins in transfected cells. Glu-Glu Tag (F3Z5J) Rabbit mAb detects exogenously expressed Glu-Glu-tagged proteins in cells expressed under a CMV promoter. Expression under other promoters has not been evaluated. This antibody may detect a band of unknown origin at 30 kDa in some rodent cell lines.
Species Reactivity:
All Species Expected
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic Glu-Glu tag peptide.
Background
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.
Limited Uses
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