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XML generation date: 2025-03-07 13:09:15.912
Product last modified at: 2025-04-03T18:15:11.113Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Glycogen Synthase (GYS1/GYS2) (15B1) Rabbit mAb #3886

Filter:
  • WB
  • IP
  • IHC
Western Blotting Image 1: Glycogen Synthase (GYS1/GYS2) (15B1) Rabbit mAb
Western blot analysis of extracts from various cell lines, using Glycogen Synthase (GYS1/GYS2) (15B1) Rabbit mAb.

To Purchase # 3886

Cat. # Size Qty. Price
3886T 20 µl
$153
3886S 100 µl
$339

Supporting Data

REACTIVITY H M R
SENSITIVITY Endogenous
MW (kDa) 84
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
  • IHC-Immunohistochemistry 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:25
Immunohistochemistry (Paraffin) 1:100

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Glycogen Synthase (GYS1/GYS2) (15B1) Rabbit mAb detects endogenous levels of total muscle and liver glycogen synthase protein.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence surrounding Val229 of human muscle glycogen synthase.

Background

Glycogen is a polysaccharide of glucose and serves as an energy storage in mammalian muscle and liver (1). Glycogen synthase catalyzes the rate-limiting step of glycogen biosynthesis and has two major isoforms in mammals: muscle isoform (glycogen synthase 1, GYS1) and liver isoform (glycogen synthase 2, GYS2), respectively (1). Glycogen synthase kinase-3α (GSK-3α) and glycogen synthase kinase-3β (GSK-3β) phosphorylate glycogen synthase at multiple sites in its C-terminus (Ser641, Ser645, Ser649, and Ser653), inhibiting its activity (2,3). Hypoxia alters glycogen metabolism including temporal changes of GYS1 expression and phosphorylation in cancer cells, suggesting the role of metabolic reprogramming of glycogen metabolism in cancer growth (1).

Pathways

Explore pathways related to this product.


For Research Use Only. Not For Use In Diagnostic Procedures.
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