Render Target: STATIC
Render Timestamp: 2024-11-21T13:21:56.019Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-09-30 01:57:58.583
Product last modified at: 2024-11-08T16:30:10.996Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

GM-CSF Receptor α (E8Z3S) Rabbit mAb #69817

Filter:
  • WB
  • IHC

    Supporting Data

    REACTIVITY
    SENSITIVITY Endogenous
    MW (kDa) 70-80
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IHC-Immunohistochemistry 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    IHC Leica Bond 1:200 - 1:800
    Immunohistochemistry (Paraffin) 1:200 - 1:800

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    For a carrier free (BSA and azide free) version of this product see product #63107.

    Protocol

    Specificity / Sensitivity

    GM-CSF Receptor α (E8Z3S) Rabbit mAb recognizes endogenous levels of total GM-CSF Receptor α protein. This antibody does not cross-react with GM-CSF Receptor β protein. Based on the antigen sequence, this antibody is predicted to recognize multiple isoforms of GM-CSF Receptor α protein, including secreted isoforms.

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human GM-CSF Receptor α protein.

    Background

    Granulocyte-macrophage colony-stimulating factor receptor subunit alpha (GM-CSF Receptor α), also known as CSF2RA and CD116, is a type 1 transmembrane protein with low affinity for GM-CSF. GM-CSF Receptor α is primarily expressed on myeloid cells, including granulocytes, monocytes, macrophages, and dendritic cells (1). GM-CSF Receptor α forms a heterodimeric receptor complex with GM-CSF Receptor β, creating the high affinity GM-CSF receptor (2). Upon dimerization and ligand binding, the β subunit becomes phosphorylated and transduces signals via Jak/STAT and MAPK pathways for cell proliferation, differentiation, and survival (3,4). Oligomerization of the high affinity GM-CSF Receptor α/β complex is required for optimal intracellular signal transduction (5). Soluble GM-CSF Receptor α, either secreted or cleaved from the cell surface, can competitively bind GM-CSF, preventing membrane receptor signaling (1,6,7). Multiple isoforms of GM-CSF Receptor α have been described.
    For Research Use Only. Not For Use In Diagnostic Procedures.
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