GNAS/Gαs (E4G1G) Rabbit mAb #47342
- WB
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 42, 45 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
There are four subclasses of Gα protein, Gαs, Gαi/o, Gαq/11, and Gα12/13 (3), and each is linked to a unique signaling pathway. Gαs is encoded by the GNAS gene. This G protein subunit is associated with a wide spectrum of disorders (4). The GTP bound Gαs activates adenylyl cyclase, which catalyzes the conversion of ATP into the second messenger cAMP and initiates the downstream PKA pathway (5). Mutations on residue Arg201 or Gln227 result in loss of GTP hydrolase activity (6), leading to constitutive activation of Gαs, which contributes to tumor progression (7,8), fibrous dysplasia of bone (9), and McCune-Albright syndrome, a disorder that causes abnormal skin pigmentation, scar tissue formation on bone, and growth-regulating gland malfunction (10). Heterozygous loss-of-function mutations in Gαs lead to Albright hereditary osteodystrophy (AHO), a disease characterized by short stature, obesity, brachydactyly, subcutaneous ossification, and dental deficits (11).
- Mafi, A. et al. (2022) Proc Natl Acad Sci USA 119, e2110085119.
- Lin, Y.L. et al. (2020) J Cancer Res Clin Oncol 146, 2179-2188.
- Chaudhary, P.K. and Kim, S. (2021) Cells 10, 3288. doi: 10.3390/cells10123288.
- Turan, S. and Bastepe, M. (2015) Curr Osteoporos Rep 13, 146-58.
- Landis, C.A. et al. (1989) Nature 340, 692-6.
- Vallar, L. (1990) Biochem Soc Symp 56, 165-70.
- Arang, N. and Gutkind, J.S. (2020) FEBS Lett 594, 4201-4232.
- Patra, K.C. et al. (2018) Nat Cell Biol 20, 811-822.
- Shin, S.J. et al. (2017) Sci Rep 7, 2836.
- Weinstein, L.S. (2006) J Bone Miner Res 21 Suppl 2, P120-4.
- Spiegel, A.M. (1997) Horm Res 47, 89-96.
Limited Uses
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