GOT2 (F4P3R) Rabbit mAb #39627
- WB
- IHC
- IF
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 41 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunohistochemistry (Paraffin) | 1:50 - 1:200 |
| Immunofluorescence (Immunocytochemistry) | 1:100 - 1:400 |
Storage
For a carrier free (BSA and azide free) version of this product see product #66911.
Protocol
Specificity / Sensitivity
GOT2 (F4P3R) Rabbit mAb recognizes endogenous levels of total GOT2 protein. This antibody does not cross-react with GOT1 protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly39 of human GOT2 protein.
Background
Glutamate oxaloacetate transaminase 2 (GOT2), a mitochondrial enzyme, catalyzes the conversion of oxaloacetate to aspartate. Studies show that the metabolite pyridoxal phosphate (PLP) is selectively required by acute myeloid leukemia. PLP-dependent GOT2 selectively sustains leukemic cell proliferation. Depletion of GOT2 suppresses the proliferation of acute myeloid leukemia cells (1). In addition, mouse hematopoietic stem cells are dependent on cell-autonomous aspartate synthesis. Got2 deletion leads to decreased aspartate levels and reduced hematopoietic stem cell function (2). Furthermore, GOT2-dependent aspartate synthesis is also necessary for protein and collagen biosynthesis in chondrocytes and is critical for chondrocyte proliferation and growth (3).
Limited Uses
Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.
Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.