Render Target: STATIC
Render Timestamp: 2024-11-25T11:07:33.214Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-09-30 01:58:50.699
Product last modified at: 2024-11-08T16:45:09.565Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

GPAT3 (E9R5I) Rabbit mAb #96164

Filter:
  • WB

    Supporting Data

    REACTIVITY M
    SENSITIVITY Endogenous
    MW (kDa) 40
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • M-Mouse 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    GPAT3 (E9R5I) Rabbit mAb recognizes endogenous levels of total GPAT3 protein.

    Species Reactivity:

    Mouse

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu397 of human GPAT3 protein.

    Background

    Glycerol-3-phosphate acyltransferases (GPATs) catalyze the first step in the de novo synthesis of triacylglycerol (TAG) and phospholipids by converting glycerol-3-phosphate to lysophosphatidic acid (LPA) (1). The GPAT family consists of four family members: two mitochondrial proteins (GPAT1 and GPAT2) and two microsomal proteins (GPAT3 and GPAT4). Each can differ in tissue expression, regulation, and physiological and pathophysiological roles (2). The microsomal proteins GPAT3 and GPAT4 were originally designated as AGPAT8 and AGPAT6, respectively. 1-acyl-glycerol-3-phosphate acyltransferase (AGPAT) catalyzes the subsequent reaction converting LPA to phosphatidic acid. The genes were renamed to GPAT3 and GPAT4 upon elucidation of GPAT activity (3,4). Both GPAT3 and GPAT4 are expressed in adipose tissue, but GPAT3 is highly induced during adipocyte differentiation (4). Brown adipose tissue (BAT) preferentially expresses GPAT4 (5). GPAT3 and GPAT4 may also be phosphorylated in response to insulin treatment to increase GPAT activity (6). Accumulation of saturated LPA produced by GPAT4 at the ER-mitochondrial contact site can inhibit autophagy through the abnormal formation of omegasomes, which are precursors to the autophagosome (7). Glycerolipid synthesis by GPAT3 and GPAT4 can also regulate activation of macrophages (8).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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