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Render Timestamp: 2024-11-28T13:03:55.207Z
Commit: d79925545b26f8827f92d145dadc6f0527debdb1
XML generation date: 2024-09-30 01:59:05.575
Product last modified at: 2024-11-14T17:15:14.473Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. GPC2 (CT3) Mouse mAb

GPC2 (CT3) Mouse mAb #90488

Filter:
  • WB
  • IHC
  • IF
  • F

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 55, 80-300
    Source/Isotype Mouse IgG1 kappa
    Application Key:
    • WB-Western Blotting 
    • IHC-Immunohistochemistry 
    • IF-Immunofluorescence 
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunohistochemistry (Paraffin) 1:200 - 1:800
    Immunofluorescence (Immunocytochemistry) 1:50 - 1:200
    Flow Cytometry (Live) 1:50 - 1:200

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    GPC2 (CT3) Mouse mAb recognizes endogenous levels of total GPC2 protein. This antibody reacts with the core GPC2 protein and glycanated forms of GPC2 protein. Based upon sequence alignment, this antibody is not predicted to cross-react with other GPC family members.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human GPC2 protein.

    Background

    Glypican 2 (GPC2) is a heparan sulfate proteoglycan that is linked to the outer leaflet of the plasma membrane via a glycosylphosphatidylinositol (GPI) linkage (1). Unlike other members of the glypican family, GPC2 is expressed in the developing nervous system, where it is thought to function as a signaling co-receptor to promote neuronal growth and axon guidance (2,3). GPC2 is considered to be an oncofetal antigen as it is highly expressed in the developing nervous system and then largely silenced in adult, normal tissues. Research studies have shown, however, that GPC2 is highly overexpressed in a high percentage of human neuroblastomas, medulloblastomas, and retinoblastomas. Given the restricted expression pattern of GPC2 in healthy, postnatal tissues, there is significant interest in targeting this antigen using a variety of immunotherapeutic approaches (4-6).

    Database Links

    UniProt ID: Q8N158


    Entrez-Gene Id: 221914


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