Render Target: STATIC
Render Timestamp: 2024-12-26T11:35:01.549Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-04-05 20:35:50.636
Product last modified at: 2024-09-26T11:45:40.186Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

GPI Antibody #57893

Filter:
  • WB

    Supporting Data

    REACTIVITY H Mk
    SENSITIVITY Endogenous
    MW (kDa) 60
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    GPI Antibody recognizes endogenous levels of total G6PI protein.

    Species Reactivity:

    Human, Monkey

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro8 of human G6PI protein. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Glucose-6-phosphate isomerase (GPI) is a multi-functional protein belonging to the glucose phosphate isomerase family (1,2). As an intracellular metabolic enzyme, GPI plays a pivotal role in glycolysis and gluconeogenesis by catalyzing the interconversion of D-glucose-6-phosphate and D-fructose-6-phosphate (3). GPI is also secreted, where it functions as a cytokine (referred to as Autocrine Motility Factor, AMF), acting via the E3-ubiquitin-protein ligase AMFR/gp78 (4). In normal tissues, GPI/AMF has been shown to promote both immune cell maturation and neuronal cell survival (5,6). It is also secreted in abundance by some tumor cells (7), where it has been shown to promote tumor cell migration and metastasis (8,9).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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