Render Target: STATIC
Render Timestamp: 2024-12-30T11:35:12.620Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-09-30 01:53:59.829
Product last modified at: 2024-12-17T18:48:28.917Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. GPR50 (D1D6I) Rabbit mAb
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

GPR50 (D1D6I) Rabbit mAb #14032

Filter:
  • WB
  • IP
  • IF

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 70-75
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50
    Immunofluorescence (Immunocytochemistry) 1:400

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    GPR50 (D1D6I) Rabbit mAb recognizes endogenous levels of total GPR50 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro420 of human GPR50 protein.

    Background

    G-protein coupled receptor 50 (GPR50) is an orphan G-protein coupled receptor with high sequence homology to the melatonin receptors MT1 and MT2. While classified as a member of the melatonin receptor family and also known as melatonin-related receptor, GPR50 does not bind melatonin (1). GPR50 forms heterodimers with MT1 and MT2 and acts as a negative regulator of MT1 agonist binding and G protein coupling; inhibition of melatonin receptor activity is dependent on the long, proline-rich carboxy terminal tail of GPR50 (2). On a physiological level, GPR50 is involved in the regulation of adaptive thermoregulation in mammals and deletion of GPR50 in mice produces a profound effect on the response to fasting and facilitates entry into torpor (3). Polymorphisms in the corresponding GPR50 gene are associated with bipolar affective disorder and major depressive disorder in women, indicating that variation in GPR50 may be an important gender-specific risk factor for certain mental disorders (4,5).

    Database Links

    UniProt ID: Q13585


    Entrez-Gene Id: 9248


    Limited Uses

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    For Research Use Only. Not For Use In Diagnostic Procedures.
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