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Commit: da7e4f2f0d1aed1f1f8e20e4e2ecab8f33cbd595
XML generation date: 2024-09-30 01:59:50.691
Product last modified at: 2025-01-01T09:03:17.311Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

GPR52 (E9D8W) Rabbit mAb #61178

Filter:
  • WB
  • IF
Western Blotting Image 1: GPR52 (E9D8W) Rabbit mAb
Western blot analysis of extracts from human cerebellum, lung, and cortex tissue using GPR52 (E9D8W) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

To Purchase # 61178

Cat. # Size Qty. Price Ships
61178T 20 µl
$156
61178S 100 µl
$371

Supporting Data

REACTIVITY H M
SENSITIVITY Endogenous
MW (kDa) 41
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IF-Immunofluorescence 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunofluorescence (Frozen) 1:50 - 1:200

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #65216.

Protocol

Specificity / Sensitivity

GPR52 (E9D8W) Rabbit mAb recognizes endogenous levels of total GPR52 protein. Nonspecific labeling of Mueller cells in fixed frozen mouse retina has been observed by immunofluorescence.

Species Reactivity:

Human, Mouse

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val249 of human GPR52 protein.

Background

G-protein coupled receptor 52 (GPR52), an orphan G-protein coupled receptor (GPCR), modulates dopaminergic and glutamatergic transmission in neuronal circuits responsible for cognitive function and emotion (1). GPR52 regulates those various brain functions through activation of cAMP-dependent pathways (2). Furthermore, GPR52 is a modulator of soluble mutant huntingtin (mHTT) levels in cells of Huntington’s disease (HD) patients (3). Therefore, GPR52 is a novel therapeutic target for HD as well as psychiatric disorders (1-4). Initial studies have shown that a novel GPR52 antagonist reduces mutant HTT levels and rescues HD-associated phenotypes in cellular and mouse models (4).
For Research Use Only. Not For Use In Diagnostic Procedures.
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