Render Target: STATIC
Render Timestamp: 2024-11-21T13:23:47.429Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-09-30 02:00:00.404
Product last modified at: 2024-09-30T08:00:58.031Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Granzyme A (E4M9S) Rabbit mAb #76135

Filter:
  • WB

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 28
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Granzyme A (E4M9S) Rabbit mAb recognizes endogenous levels of total Granzyme A protein. This antibody does not cross-react with human Granzyme B, H, K, or M proteins.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu115 of human Granzyme A protein.

    Background

    Granzymes are a family of serine proteases expressed by cytotoxic T lymphocytes and natural killer (NK) cells and are key components of immune responses to pathogens and transformed cells (1). Granzymes are synthesized as zymogens and are processed into mature enzymes by cleavage of a leader sequence. They are released by exocytosis in lysosome-like granules containing perforin, a membrane pore-forming protein (2). Granzyme A, or GrA, is a dominant mediator of toxicity in vitro, and is the most abundant protease in cytotoxic granules (3). Granzyme A cleaves substrate after Arg or Lys and can activate caspase-independent cell death pathways upon cleavage of the mitochondrial protein NDUFS3. Cleavage of NDUFS3 leads to reactive oxygen species (ROS) generation, which triggers translocation of the SET complex to the nucleus, where Granzyme A cleaves the SET complex and leads to the opening of DNA and DNA nicks (4-7).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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    KARPAS cell line source: Dr. Abraham Karpas at the University of Cambridge.
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